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A Two‐Color, Self‐Controlled Molecular Beacon
Author(s) -
Biner Sarah M.,
Häner Robert
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100651
Subject(s) - chromophore , fluorescence , computer science , control (management) , feature (linguistics) , pyrene , chemistry , acceptor , information retrieval , combinatorial chemistry , computational biology , photochemistry , artificial intelligence , biology , physics , organic chemistry , optics , linguistics , philosophy , condensed matter physics
Control yourself! A two‐color molecular beacon with non‐nucleosidic chromophores in a triplex stem is presented. Pyrene and PDI fluorophores act as mutual quenchers by formation of a donor–acceptor complex in the closed form. Hybridization with the target results in two independent fluorescence signals. The two‐color read‐out provides a “self‐control” feature, which helps to eliminate false positive signals in imaging and screening applications.