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Expansion of the Amino Acid Repertoire in Protein Biosynthesis in Silkworm Cells
Author(s) -
Teramoto Hidetoshi,
Kojima Katsura,
Kajiwara Hideyuki,
Ishibashi Jun
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100624
Subject(s) - aminoacyl trna synthetase , amino acid , biochemistry , biosynthesis , protein biosynthesis , protein engineering , mutant , bombyx mori , aminoacylation , green fluorescent protein , chemistry , transfer rna , substrate specificity , biology , enzyme , gene , rna
Toward silk production with UAAs : The strategy for residue‐specific incorporation of unnatural amino acids (UAAs) into proteins was extended to silkworm cells. Here we report the incorporation of p ‐Cl‐Phe into a reporter protein (EGFP) synthesized in silkworm cells that express an αA450G mutant of B. mori phenylalanyl‐tRNA synthetase with relaxed substrate specificity.