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Kinetic Capillary Electrophoresis with Mass‐Spectrometry Detection (KCE‐MS) Facilitates Label‐Free Solution‐Based Kinetic Analysis of Protein–Small Molecule Binding
Author(s) -
Bao Jiayin,
Krylova Svetlana M.,
Wilson Derek J.,
Reinstein Oren,
Johnson Philip E.,
Krylov Sergey N.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100617
Subject(s) - capillary electrophoresis , small molecule , mass spectrometry , tandem , tandem mass spectrometry , chromatography , kinetics , combinatorial chemistry , chemistry , computational biology , biochemistry , biology , physics , quantum mechanics , composite material , materials science
Tandem tracker: Here we introduce a method for studying the kinetics of protein–small‐molecule interactions based on kinetic capillary electrophoresis (KCE) separation and MS detection. Due to the variety of KCE methods and MS modes available, the KCE‐MS tandem is a highly versatile platform for label‐free, solution‐based kinetic studies of affinity interactions.