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Bromomaleimide‐Linked Bioconjugates Are Cleavable in Mammalian Cells
Author(s) -
Moody Paul,
Smith Mark. E. B.,
Ryan Chris P.,
Chudasama Vijay,
Baker James R.,
Molloy Justin,
Caddick Stephen
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100603
Subject(s) - chemistry , förster resonance energy transfer , cleave , biomolecule , cytoplasm , rhodamine , bioconjugation , cleavage (geology) , combinatorial chemistry , cysteine , biophysics , fluorescence , biochemistry , enzyme , materials science , biology , physics , composite material , quantum mechanics , fracture (geology)
Bromomaleimides are versatile scaffolds that allow facile conjugation of thiolated biomolecules. Here we demonstrate that bromomaleimide‐linked GFP–rhodamine FRET pairs cleave in the cytoplasm of mammalian cells. We believe that bromomaleimide scaffolds provide a potential core structure for prodrugs designed to release bioactive cargo following cell internalisation.