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Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25
Author(s) -
Pan Si Jia,
Rajniak Jakub,
Cheung Wai Ling,
Link A. James
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100596
Subject(s) - enzyme , function (biology) , mutagenesis , directed evolution , computational biology , biochemistry , peptide , transporter , chemistry , atp binding cassette transporter , gene , biology , computer science , genetics , mutation , mutant
Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter (see graphic). We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.