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Mechanism‐Oriented Redesign of an Isomaltulose Synthase to an Isomelezitose Synthase by Site‐Directed Mutagenesis
Author(s) -
Görl Julian,
Timm Malte,
Seibel Jürgen
Publication year - 2012
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100576
Subject(s) - atp synthase , mutagenesis , isomerase , chemistry , biochemistry , site directed mutagenesis , enzyme , biocatalysis , yield (engineering) , sucrose synthase , nutraceutical , mutation , catalysis , reaction mechanism , mutant , gene , materials science , metallurgy , invertase
An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6‐ O F ‐glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.