Mechanism‐Oriented Redesign of an Isomaltulose Synthase to an Isomelezitose Synthase by Site‐Directed Mutagenesis | Zendy

Görl Julian | Zendy; Timm Malte | Zendy; Seibel Jürgen | Zendy

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Mechanism‐Oriented Redesign of an Isomaltulose Synthase to an Isomelezitose Synthase by Site‐Directed Mutagenesis

Author(s) -

Görl Julian,

Timm Malte,

Seibel Jürgen

Publication year - 2012

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201100576

Subject(s) - atp synthase , mutagenesis , isomerase , chemistry , biochemistry , site directed mutagenesis , enzyme , biocatalysis , yield (engineering) , sucrose synthase , nutraceutical , mutation , catalysis , reaction mechanism , mutant , gene , materials science , metallurgy , invertase

An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6‐ O F ‐glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.

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