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Rational Design of Fluorescent Biosensor for Cyclic di‐GMP
Author(s) -
Ho Chun Loong,
Koh Siew Lee,
Chuah Mary Lay Cheng,
Luo Zhen,
Tan Wei Jian,
Low Donovan Kian Soon,
Liang ZhaoXun
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100557
Subject(s) - biosensor , fluorophore , second messenger system , chemistry , fluorescence , enzyme , rational design , phosphodiesterase , biochemistry , biophysics , combinatorial chemistry , biology , nanotechnology , materials science , physics , quantum mechanics
Messenger bagged: The design of a fluorophore‐labeled protein biosensor for the bacterial messenger cyclic di‐GMP is described. The biosensor responds to c‐di‐GMP with sub‐micromolar sensitivity in a real‐time fashion. The biosensor can be used for enzyme assays for diguanylate cyclases and c‐di‐GMP phosphodiesterases as well as the high‐throughput screening of inhibitors.