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Investigating Mithramycin Deoxysugar Biosynthesis: Enzymatic Total Synthesis of TDP‐ D ‐Olivose
Author(s) -
Wang Guojun,
Kharel Madan K.,
Pahari Pallab,
Rohr Jürgen
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100540
Subject(s) - biosynthesis , enzyme , chemistry , biochemistry , bifunctional , phosphofructokinase 2 , cofactor , stereochemistry , catalysis
Mix'n'match: Enzymatic total synthesis of TDP‐ D ‐olivose was achieved, starting from TDP‐4‐keto‐6‐deoxy‐ D ‐glucose, by combining three pathway enzymes with one cofactor‐regenerating enzyme. The results also revealed that MtmC is a bifunctional enzyme that can perform a 4‐ketoreduction necessary for D ‐olivose biosynthesis besides the previously found C ‐methyltransfer for D ‐mycarose biosynthesis.
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