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SEM Observation of Wet Biological Specimens Pretreated with Room‐Temperature Ionic Liquid
Author(s) -
Tsuda Tetsuya,
Nemoto Noriko,
Kawakami Koshi,
Mochizuki Eiko,
Kishida Shoko,
Tajiri Takako,
Kushibiki Toshihiro,
Kuwabata Susumu
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100476
Subject(s) - ionic liquid , scanning electron microscope , materials science , nanotechnology , chemical engineering , ionic bonding , biological specimen , biomedical engineering , chemistry , ion , composite material , organic chemistry , optics , physics , engineering , catalysis , medicine
A facile pretreatment process for SEM: The use of room temperature ionic liquids (RTILs) provides an interesting method for SEM of biological specimens. We used a novel and concise method of pretreatment, excluding fixation or Au sputtering steps. Fine and smooth‐textured SEM images of a wide variety of biological specimens treated in this way were observed without artefacts.