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Semisynthesis of Prenylated Rab GTPases by Click Ligation
Author(s) -
Yi Long,
Abootorabi Mostafa,
Wu YaoWen
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100466
Subject(s) - semisynthesis , rab , gtpase , prenylation , click chemistry , computer science , computational biology , chemistry , biochemistry , biology , combinatorial chemistry , enzyme
Click on the link: Tandem expressed protein ligation (EPL) and click reaction provide access to functional geranylgeranylated Rab GTPases (see scheme). The reactions are quantitative and highly efficient. This ligation strategy could open up a new avenue for lipidated protein production and provide pure post‐translationally modified lipidated proteins for various biological studies.