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Photoactivatable Caged Cyclic RGD Peptide for Triggering Integrin Binding and Cell Adhesion to Surfaces
Author(s) -
Wirkner Melanie,
Weis Simone,
San Miguel Verónica,
Álvarez Marta,
Gropeanu Radu A.,
Salierno Marcelo,
Sartoris Anne,
Unger Ronald E.,
Kirkpatrick C. James,
del Campo Aránzazu
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100437
Subject(s) - integrin , quartz crystal microbalance , chemistry , biophysics , cell adhesion , peptide , adhesion , biocompatibility , cell , rgd motif , in situ , microbiology and biotechnology , biochemistry , biology , organic chemistry , adsorption
We report the synthesis and properties of a photoactivatable caged RGD peptide and its application for phototriggering integrin‐ and cell‐binding to surfaces. We analysed in detail 1) the differences in the integrin‐binding affinity of the caged and uncaged forms by quartz crystal microbalance (QCM) studies, 2) the efficiency and yield of the photolytic uncaging reaction, 3) the biocompatibility of the photolysis by‐products and irradiation conditions, 4) the possibility of site, temporal and density control of integrin‐binding and therefore human cell attachment, and 5) the possibility of in situ generation of cell patterns and cell gradients by controlling the UV exposure. These studies provide a clear picture of the potential and limitations of caged RGD for integrin‐mediated cell adhesion and demonstrate the application of this approach to the control and study of cell interactions and responses.