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An Acyloxymethyl Ketone‐Based Probe to Monitor the Activity of Glutathionylspermidine Amidase in Escherichia coli
Author(s) -
Chong CheongMeng,
Gao Shijay,
Chiang BingYu,
Hsu WenHung,
Lin TingChien,
Chen TzuChieh,
Lin ChunHung
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100417
Subject(s) - amidase , escherichia coli , ketone , chemistry , biochemistry , redox , combinatorial chemistry , organic chemistry , enzyme , gene
Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone‐based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal‐induced stress.