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Mutant Malonyl‐CoA Synthetases with Altered Specificity for Polyketide Synthase Extender Unit Generation
Author(s) -
Koryakina Irina,
Williams Gavin J.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100383
Subject(s) - polyketide , polyketide synthase , mutant , mutagenesis , directed evolution , biochemistry , saturated mutagenesis , computational biology , enzyme , biology , chemistry , biosynthesis , gene
Tailoring guide: We have used structure‐guided saturation mutagenesis followed by colorimetric screening to identify mutant malonyl‐CoA synthetases with altered substrate specificity. One particular mutant displayed a 240‐fold shift in specificity (see graphic). These mutant enzymes will be useful tools for providing extender units to probe the activity of polyketide synthases.