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Correlated Optical and Electrical Single‐Molecule Measurements Reveal Conformational Diffusion from Ligand Binding to Channel Gating in the Nicotinic Acetylcholine Receptor
Author(s) -
Schmauder Ralf,
Kosanic Davor,
Hovius Ruud,
Vogel Horst
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100302
Subject(s) - gating , channel (broadcasting) , agonist , nicotinic acetylcholine receptor , chemistry , diffusion , biophysics , event (particle physics) , acetylcholine receptor , mechanism (biology) , computer science , receptor , telecommunications , physics , biochemistry , biology , quantum mechanics , thermodynamics
Towards channel opening: The time delay between binding of a fluorescent agonist and the subsequent gating event was evaluated directly from optical and electrical single‐event traces measured on an individual receptor. The distribution of such time delays was best fitted by a 3/2 power‐law, indicating that conformational diffusion was the channel‐activation mechanism.