Premium
Generating DNA Synbodies from Previously Discovered Peptides
Author(s) -
Liu Rui,
Jiang Bing,
Yu Hanyang,
Chaput John C.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100284
Subject(s) - hela , dna , chemistry , peptide , combinatorial chemistry , phage display , antibody , computational biology , biochemistry , protein engineering , microbiology and biotechnology , cell , biology , enzyme , genetics
Synbody building: We demonstrate how off‐the‐shelf peptides can be transformed into high affinity protein capture reagents that mimic the recognition properties of natural antibodies (see figure). The designer synthetic antibody amplifies the binding affinity of the individual peptides by ∼1000‐fold to bind Grb2 with a K d of 2 n M , and functions with high selectivity in conventional pull‐down assays from HeLa cell lysates.