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Subcellular Protein Localization by Using a Genetically Encoded Fluorescent Amino Acid
Author(s) -
Charbon Godefroid,
Brustad Eric,
Scott Kevin A.,
Wang Jiangyun,
LøbnerOlesen Anders,
Schultz Peter G.,
JacobsWagner Christine,
Chapman Eli
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100282
Subject(s) - ftsz , fluorescence , fluorescent protein , cleavage (geology) , amino acid , biochemistry , subcellular localization , green fluorescent protein , protein subcellular localization prediction , biology , cell , yellow fluorescent protein , microbiology and biotechnology , cell division , chemistry , gene , physics , quantum mechanics , paleontology , fracture (geology)
CouAA‐lescence: A fluorescent amino acid (CouAA) was site‐specifically incorporated into FtsZ, the bacterial tubulin homologue, in living cells. FtsZ CouAA is seen at the cleavage furrow during cell division to form the Z‐ring. These studies provide the first example of a fully functional protein to be visualized in vivo by using a genetically incorporated fluorescent amino acid.