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Long‐Range Distance Determination in a DNA Model System inside Xenopus laevis Oocytes by In‐Cell Spin‐Label EPR
Author(s) -
Azarkh Mykhailo,
Okle Oliver,
Singh Vijay,
Seemann Isabelle T.,
Hartig Jörg S.,
Dietrich Daniel R.,
Drescher Malte
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100281
Subject(s) - electron paramagnetic resonance , xenopus , site directed spin labeling , dna , computational biology , spin (aerodynamics) , range (aeronautics) , biophysics , computer science , nanotechnology , chemistry , information retrieval , biology , gene , physics , biochemistry , nuclear magnetic resonance , materials science , composite material , thermodynamics
DEER strides: The development of analytical tools for elucidating native structures inside cells is an ongoing challenge. Conformational changes of DNA upon injection into living cells (see figure) can be detected by a combination of site‐directed spin labeling and DEER (double electron–electron resonance) spectroscopy. This technique offers structural information on the nm scale, in cellulo, background‐free and at low concentrations.