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Detection of 5‐Hydroxymethylcytosine in DNA by Transferring a Keto‐Glucose by Using T4 Phage β‐Glucosyltransferase
Author(s) -
Song ChunXiao,
Sun Yao,
Dai Qing,
Lu XingYu,
Yu Miao,
Yang CaiGuang,
He Chuan
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100278
Subject(s) - glucosyltransferase , linker , 5 hydroxymethylcytosine , dna , computational biology , chemistry , biochemistry , combinatorial chemistry , computer science , biology , gene , programming language , dna methylation , gene expression
Capture with ketone: 5‐Hydroxylmethylcytosine (5‐hmC) in DNA can be selectively labeled with a keto‐glucose and subsequently linked to biotin via a ketoxime linker for enrichment and detection/sequencing purposes (see scheme). Keto‐glucose can be more efficiently transferred than azide‐glucose by β‐glucosyltransferase, and can provide single‐base resolution detection/ sequencing of 5‐hmC.
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