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A BODIPY‐Cyclooctyne for Protein Imaging in Live Cells
Author(s) -
Beatty Kimberly E.,
Szychowski Janek,
Fisk John D.,
Tirrell David A.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100277
Subject(s) - bodipy , fluorophore , flow cytometry , fluorescence microscope , biophysics , fluorescence lifetime imaging microscopy , chemistry , azide , fluorescence , microbiology and biotechnology , microscopy , live cell imaging , intracellular , biology , biochemistry , cell , pathology , medicine , physics , organic chemistry , quantum mechanics
Cellular proteins that bear reactive azides can be imaged by fluorescence microscopy following strain‐promoted ligation to cyclooctyne dyes. Here we describe BODIPY‐cyclooctyne (BDPY), a membrane‐permeant fluorophore that can be used to label intracellular proteins in live mammalian cells. Flow cytometry reveals fluorescence signals more than 25‐fold above background after labeling of azide‐tagged cells with BDPY.