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Glycosynthase‐Mediated Assembly of Xylanase Substrates and Inhibitors
Author(s) -
GoddardBorger Ethan D.,
Fiege Brigitte,
Kwan Emily M.,
Withers Stephen G.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100229
Subject(s) - xylanase , chemistry , glycoside hydrolase , nanotechnology , computational biology , biochemistry , biology , enzyme , materials science
Abstract An exo ‐ β ‐xylosidase mutant with glycosynthase activity was created to aid in the synthesis of xylanase substrates and inhibitors. Simple monosaccharides were easily elaborated into di‐, tri‐ and tetrasaccharides by using this enzyme. Some products proved to be surprisingly potent inhibitors of xylanases from glycoside hydrolase families 10 and 11.