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Protein Modification by Strain‐Promoted Alkyne–Azide Cycloaddition
Author(s) -
van Hest Jan C. M.,
van Delft Floris L.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100206
Subject(s) - bioorthogonal chemistry , cycloaddition , azide , strain (injury) , alkyne , chemistry , genetic code , protein engineering , click chemistry , combinatorial chemistry , chemical modification , enzyme , biochemistry , organic chemistry , biology , amino acid , catalysis , anatomy
Taking the strain: Strain‐promoted cycloadditions between alkynes and azides have facilitated mild and bioorthogonal protein modification. Azides can be introduced into a protein by a variety of chemical, enzymatic or genetic techniques. Now genetic encoding of a cyclooctyne offers new opportunities for protein modification and the study of proteins in their native habitat.