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Probing Protein–Protein Interactions with a Genetically Encoded Photo‐crosslinking Amino Acid
Author(s) -
Ai Huiwang,
Shen Weijun,
Sagi Amit,
Chen Peng R.,
Schultz Peter G.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100194
Subject(s) - amino acid , transfer rna , diazirine , biochemistry , chemistry , substrate (aquarium) , chemical biology , biology , gene , rna , ecology
A pyrrolysyl tRNA/tRNA sythetase pair has been evolved that efficiently incorporates an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells. When site‐specifically introduced into the active site of Cdk5, this unnatural amino acid was able to photo‐crosslink the kinase to its substrate, Pak1.