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Transition State of Rare Event Base Pair Opening Probed by Threading into Looped DNA
Author(s) -
Kogan Maxim,
Nordén Bengt,
Lincoln Per,
Nordell Pär
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100182
Subject(s) - threading (protein sequence) , dna , thread (computing) , base pair , duplex (building) , nanotechnology , chemistry , crystallography , materials science , computer science , protein structure , biochemistry , operating system
Thread with care: The threading of a dumb‐bell shaped ruthenium complex between two DNA strands (see figure) is dependent on rare, spontaneous duplex openings. We have investigated DNA constructs structurally mimicking hypothetical transition states, in which threading, and unthreading, proved to be greatly facilitated.