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FIT for Purpose: PNA‐Based Probes Enable mRNA Imaging in Living Cells
Author(s) -
Tilsner Jens,
Flors Cristina
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100139
Subject(s) - peptide nucleic acid , fluorescence , nucleic acid , messenger rna , fluorescence microscope , in vivo , biophysics , chemistry , intercalation (chemistry) , microbiology and biotechnology , biochemistry , nanotechnology , biology , materials science , genetics , optics , physics , gene , organic chemistry
If the dye FITs: A new approach for imaging mRNA in vivo is based on forced intercalation (FIT) of a dye that substitutes a base of a peptide nucleic acid hybridisation probe. Upon hybridisation with complementary mRNA, the fluorescence of the dye is greatly enhanced, improving the signal‐to‐noise ratio for fluorescence microscopy in living cells.