Premium
A Ribonucleotide Reductase‐Like Electron Transfer System in the Nitroaryl‐Forming N‐Oxygenase AurF
Author(s) -
Fries Alexander,
Bretschneider Tom,
Winkler Robert,
Hertweck Christian
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100138
Subject(s) - ribonucleotide reductase , ferredoxin , ribonucleotide , electron transfer , chemistry , mutagenesis , reductase , stereochemistry , biophysics , biochemistry , biology , enzyme , photochemistry , gene , mutation , nucleotide , protein subunit
Nitro, electrons, and RNR. Through structure comparisons, mutagenesis, and modeling we deduced that the unusual nitro‐synthase AurF docks to ferredoxin and transfers electrons from the surface to the active site across hydrogen bonds in analogy to ribonucleotide reductases (RNRs). Recruitment of RNR electron‐transfer components could rationalize the successful in vivo reconstitution of AurF in different expression hosts and its growth‐phase‐dependent activity.