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Colorimetric SNP Genotyping Based on Allele‐Specific PCR by Using a Thiol‐Labeled Primer
Author(s) -
Jung Ye Lim,
Jung Cheulhee,
Parab Harshala,
Cho DaeYeon,
Park Hyun Gyu
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100098
Subject(s) - genotyping , primer (cosmetics) , polymerase chain reaction , snp , computational biology , single nucleotide polymorphism , genetics , snp genotyping , molecular inversion probe , allele , microbiology and biotechnology , biology , gene , genotype , chemistry , organic chemistry
Red gold/blue gold: A highly convenient colorimetric method for the identification of single‐nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele‐specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the BRCA1 gene.