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An Engineered Methionyl‐tRNA Synthetase Enables Azidonorleucine Incorporation in Methionine Prototrophic Bacteria
Author(s) -
Abdeljabbar Diya M.,
Klein Thomas J.,
Link A. James
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100089
Subject(s) - auxotrophy , biology , plasmid , methionine , transfer rna , recombinant dna , bacteria , gene , genetics , biochemistry , computational biology , escherichia coli , amino acid , rna
Have you Met this? Methionine prototrophic E. coli harboring a single genomic copy of an engineered methionyl‐tRNA synthetase (MetRS) gene, metG* , are more efficient at incorporating azidonorleucine (ANL) into recombinant proteins than prototrophic E. coli containing a multicopy plasmid expressing metG* and a genomic copy of the native MetRS ( metG ).
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