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Development of an Affinity‐Based Proteomic Strategy for the Elucidation of Proanthocyanidin Biosynthesis
Author(s) -
Chalumeau Céline,
Deffieux Denis,
Chaignepain Stéphane,
Quideau Stéphane
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100044
Subject(s) - biosynthesis , proanthocyanidin , computational biology , identification (biology) , biochemistry , affinity chromatography , chemistry , biology , gene , polyphenol , enzyme , botany , antioxidant
Affinity and beyond! An affinity chromatography tool based on specific flavanol–protein interactions has been developed to allow the selective purification and identification by proteomic analysis of functional proteins involved in the last steps of proanthocyanidin biosynthesis.