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Cell‐Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β‐Lactamase‐Tag Technology
Author(s) -
Yoshimura Akimasa,
Mizukami Shin,
Hori Yuichiro,
Watanabe Shuji,
Kikuchi Kazuya
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201100021
Subject(s) - biotinylation , photobleaching , streptavidin , chemistry , internalization , fluorescence recovery after photobleaching , fluorescence , biophysics , fluorescence microscope , biotin , nanotechnology , cell , biochemistry , materials science , membrane , biology , physics , quantum mechanics
Time‐lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β‐lactamase tag (BL‐tag) technology for tethering target proteins and streptavidin‐conjugated luminescent nanoparticles. This method enabled visualization of BL‐tag‐fused epidermal growth factor receptor (EGFR) internalization and pulse‐chase labeling by using three kinds of quantum dots (QDs).