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Inside Cover: Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site‐Saturated Libraries (ChemBioChem 17/2010)
Author(s) -
Hulley Martyn E.,
Toogood Helen S.,
Fryszkowska Anna,
Mansell David,
Stephens Gill M.,
Gardiner John M.,
Scrutton Nigel S.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201090083
Subject(s) - pentaerythritol tetranitrate , cover (algebra) , pentaerythritol , chemistry , active site , directed evolution , mutant , enzyme , biochemistry , organic chemistry , engineering , mechanical engineering , fire retardant , gene , explosive material
The inside cover picture shows the crystal structure and high‐throughput screening procedure of pentaerythritol tetranitrate reductase (PETNR) for generating biocatalysts with altered product identity and/or enantiopurities. The lower figure shows the active site and altered biocatalytic abilities of wild‐type and mutant PETNR. For more information, see the paper by N. S. Scrutton et al. on p. 2433 ff.