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Cover Picture: Live‐Cell Imaging with Water‐Soluble Aminophenoxazinone Dyes Synthesised through Laccase Biocatalysis (ChemBioChem 10/2010)
Author(s) -
Bruyneel Frédéric,
D'Auria Ludovic,
Payen Olivier,
Courtoy Pierre. J.,
MarchandBrynaert Jacqueline
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201090043
Subject(s) - endosome , chemistry , fluorescence , biocatalysis , endocytosis , organelle , biophysics , protonation , live cell imaging , biochemistry , combinatorial chemistry , cell , organic chemistry , biology , catalysis , reaction mechanism , ion , physics , quantum mechanics
The cover picture shows the molecular structure of compound # 8 c from a novel family of water‐soluble sulfonated aminophenoxazinone dyes, which have been synthesised by laccase‐catalysed oxidative cyclisation. Its aromatic rings emit green fluorescent light. A cell‐biological application is illustrated by vital imaging in either transmission mode (light background) or confocal fluorescence (dark background). Being membrane‐impermeant , compound # 8 c is internalised by vesicular uptake (endocytosis) towards lysosomes. Lysosomes are simultaneously labelled by LysoTracker Red, a membrane‐permeable weak base that accumulates by protonation sequestration. Upon docking of endosomes labelled with compound # 8 c onto lysosomes, partial superimposition of the green signal from incoming organelles onto the red lysosomal signal appears as yellow light. These data illustrate the penultimate stage of the degradative pathway to lysosomes. For further information, see the paper by J. Marchand‐Brynaert et al. on p. 1451 ff.