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Chemical Synthesis of a Bisphosphorylated Mannose‐6‐Phosphate N‐Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging
Author(s) -
Liu Yunpeng,
Chan Yan Mei,
Wu Jianhui,
Chen Chen,
Benesi Alan,
Hu Jing,
Wang Yanming,
Chen Gong
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000785
Subject(s) - glycan , carbonic anhydrase ii , internalization , carbonic anhydrase , fluorescence , chemistry , biochemistry , mannose , in vivo , enzyme , glycoprotein , cell , biology , physics , microbiology and biotechnology , quantum mechanics
Red‐ily made: The first chemical synthesis of a fully elaborated bisphosphorylated triantennary M6P N‐glycan was achieved through a highly efficient late‐stage phosphorylation strategy. A human carbonic anhydrase II‐based fluorescently tagged neoglycoprotein platform was successfully constructed in order to evaluate the M6P N‐glycan‐directed protein internalization process in cell‐based assays by confocal fluorescence imaging.