Premium
Multiple‐Catalytic Sensing of Nucleic Acid Sequences by Utilising a DNA–RNA–DNA Chimeric Antisense Probe and RNase H with a Eukaryotic Cell‐Free Translation System
Author(s) -
Ogawa Atsushi
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000744
Subject(s) - rnase h , nucleic acid , dna , rna , rnase p , microbiology and biotechnology , sense (electronics) , biology , nick translation , nucleic acid thermodynamics , translation (biology) , hybridization probe , chemistry , messenger rna , biochemistry , gene
Double sense: A novel multiple‐catalytic DNA sensing method utilises a chimeric probe that targets both the DNA and reporter mRNA. The technique uses just probe, RNase H, luciferase mRNA and wheat germ extract. With its four discrete catalytic processes including cycling probe technology, this method shows relatively high sensitivity and specificity despite requiring neither labelling nor nucleic acid amplification.