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Intense Blue Fluorescence in a Leucine Zipper Assembly
Author(s) -
Tsutsumi Hiroshi,
Abe Seiichiro,
Mino Tomoaki,
Nomura Wataru,
Tamamura Hirokazu
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000692
Subject(s) - leucine zipper , zipper , fluorescence , chemistry , leucine , stereochemistry , biochemistry , amino acid , peptide sequence , physics , algorithm , quantum mechanics , computer science , gene
Fluorescence switching of 7‐diethylaminocoumarin‐3‐carboxylic acid (DEAC) from a dark state to bright blue was achieved through the formation of a 3α‐helical bundle structure. The DEAC unit in the leucine zipper assembly showed fluorescence 50 times stronger than that of the DEAC unit alone. The stable 3α‐helical bundle structure was essential for the great enhancement of the DEAC fluorescence.