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Universal Sensing by Transduction of Antibody Binding with Backscattering Interferometry
Author(s) -
Kussrow Amanda,
Baksh Michael M.,
Bornhop Darryl J.,
Finn M. G.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000671
Subject(s) - interferometry , computational biology , trace (psycholinguistics) , antibody , computer science , refractive index , range (aeronautics) , chemistry , biological system , biophysics , biology , materials science , optics , physics , genetics , philosophy , linguistics , composite material
All binding events induce a change in refractive index, and antibody–antigen interactions are no exception. We describe the use of backscattering interferometry to quantify antibody binding without labels and with high sensitivity. As a broad range of antibodies are available, this method represents a general way to selectively detect a wide variety of trace molecules in simple or complex mixtures.