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Dissecting Cell Signaling Pathways with Genetically Encoded 3‐Iodo‐ L ‐tyrosine
Author(s) -
Hayashi Akiko,
Hino Nobumasa,
Kobayashi Takatsugu,
Arai Ryoichi,
Shirouzu Mikako,
Yokoyama Shigeyuki,
Sakamoto Kensaku
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000665
Subject(s) - tyrosine , proteome , tyrosine phosphorylation , phosphorylation , computational biology , genetic code , amino acid , biology , signal transduction , biochemistry , chemistry
Marking the proteome: A single amino acid residue in the proteome was labeled by mammalian genetic code expansion, which incorporated 3‐iodotyrosine at a particular tyrosine phosphorylation site in cell‐signaling pathways. Phosphorylation at the site was analyzed by an antibody specific for the tyrosine derivative.