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Nondenaturing Chemical Proteomics for Protein Complex Isolation and Identification
Author(s) -
Budin Ghyslain,
MouneDimala Martin,
Leriche Geoffray,
Saliou JeanMichel,
Papillon Julie,
SanglierCianférani Sarah,
Van Dorsselaer Alain,
Lamour Valérie,
Brino Laurent,
Wagner Alain
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000574
Subject(s) - cleavage (geology) , linker , computational biology , chemistry , dna , proteomics , biochemistry , identification (biology) , combinatorial chemistry , computer science , biology , gene , botany , paleontology , fracture (geology) , operating system
Controlled fishing: Mild cleavage conditions enabled by the use of the HAZA linker allowed smooth release of captured proteins under nondenaturing conditions. A DNA gyrase complex A2B2 was enriched then released in native form by chemical cleavage while preserving its enzymatic activity. This procedure allows the capture and release of endogenous protein complexes under native conditions.