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Combinatorial Self‐Assembly of Glycan Fragments into Microarrays
Author(s) -
Huang KuoTing,
Gorska Katarzyna,
Alvarez Susana,
Barluenga Sofia,
Winssinger Nicolas
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000567
Subject(s) - glycan , cooperativity , nucleic acid , computational biology , chemistry , dna microarray , biochemistry , combinatorial chemistry , biology , gene , glycoprotein , gene expression
Complex glycan arrays: A general method for preparing thioglycosides from native oligosaccharides and coupling them to peptide nucleic acids (PNAs) is reported. An array of 625 glycans was assembled by hybridization of two PNA‐encoded libraries of 25 glycan fragments. The cooperativity of the fragment for carbohydrate binding proteins was validated with lectins of known specificity.