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A Library Approach for the Discovery of Customized Yeast Three‐Hybrid Counter Selections
Author(s) -
Wingler Laura M.,
Cornish Virginia W.
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000543
Subject(s) - yeast , synthetic biology , reporter gene , computational biology , selection (genetic algorithm) , gene , biology , genetics , computer science , artificial intelligence , gene expression
Developing effective new reporter genes for synthetic biology: Here we demonstrate that we can readily develop a robust counter selection for the yeast three‐hybrid assay by screening libraries of genes not traditionally used as reporters, rather than by adapting classic reporter genes.