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Implantation of Post‐translational Tyrosylprotein Sulfation into a Prokaryotic Expression System
Author(s) -
Lu LuYi,
Chen BoHan,
Wu Jennifer YunShin,
Wang ChenChu,
Chen DaHuang,
Yang YuhShyong
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000540
Subject(s) - sulfation , tyrosine , biochemistry , sulfotransferase , chemistry , computational biology , in vivo , in vitro , catalysis , biology , microbiology and biotechnology
Sulfation made easy: Syntheses of tyrosine‐sulfated proteins were developed by coupling an in situ 3′‐phosphoadenosine‐5′‐phosphosulfate (PAPS)‐generating system and tyrosylprotein sulfotransferase (TPST) catalysis. High catalytic efficiency of TPST in vitro was demonstrated, and by using a similar strategy, sulfated proteins were produced in vivo through bacterial cultivation.