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Enzyme‐Catalyzed Substrate Attachment to Phage Surfaces for the Selection of Catalytic Activities
Author(s) -
Sunbul Murat,
Emerson Nyssa,
Yin Jun
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000475
Subject(s) - phage display , phagemid , enzyme , selection (genetic algorithm) , substrate (aquarium) , catalysis , chemistry , substrate specificity , combinatorial chemistry , bacteriophage , biochemistry , stereochemistry , biology , computer science , gene , escherichia coli , peptide , ecology , artificial intelligence
Catalysis‐based phage selection: A phage selection method has been developed to allow the codisplay of enzymes and their substrate molecules on phage surface. Substrate turnover by phage‐displayed enzymes affords product‐attached phages that can be enriched by affinity selection. Such a selection method can be a useful platform for identifying desired enzymatic activities in a phage library.