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Enzymatic Cyclisation of Peptides with a Transglutaminase
Author(s) -
Touati Jeremy,
Angelini Alessandro,
Hinner Marlon J.,
Heinis Christian
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000451
Subject(s) - tissue transglutaminase , glutamine , lysine , linker , enzyme , biochemistry , peptide , chemistry , residue (chemistry) , amino acid , computer science , programming language
The ability of microbial transglutaminase to ligate two polypeptides through glutamine and lysine residues was exploited to generate cyclic peptides. Peptides with an N‐terminal short glutamine‐donor sequence (Ala‐Leu‐Gln), a variable polypeptide linker and a C‐terminal lysine residue could efficiently be cyclised by the enzyme.