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A Novel Fungal Flavin‐Dependent Halogenase for Natural Product Biosynthesis
Author(s) -
Zeng Jia,
Zhan Jixun
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000439
Subject(s) - biosynthesis , natural product , chemistry , biocatalysis , enzyme , flavin group , biochemistry , stereochemistry , substrate (aquarium) , combinatorial chemistry , biology , catalysis , reaction mechanism , ecology
A novel halogenating agent: A fungal halogenase Rdc2 from Pochonia chlamydosporia has been reconstituted in E. coli and biochemically characterized as the dedicated halogenase in radicicol biosynthesis. The enzyme displays broad substrate specificity towards molecules such as dihydroresorcylide ( 1 ) to generate mono‐ and dihalogenated (X=Br or Cl) derivatives, thus representing a promising biocatalyst for natural product biosynthesis.