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Esterification of an Unnatural Amino Acid Structurally Deviating from Canonical Amino Acids Promotes Its Uptake and Incorporation into Proteins in Mammalian Cells
Author(s) -
Takimoto Jeffrey K.,
Xiang Zheng,
Kang JiYong,
Wang Lei
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000436
Subject(s) - amino acid , intracellular , chemistry , transfer rna , biochemistry , computational biology , combinatorial chemistry , stereochemistry , biology , rna , gene
It's a cover up: Unnatural amino acids (UAAs) with noncanonical side chains can be efficiently transported into mammalian cells when the carboxyl group of the UAA is masked with selected ester groups. This greatly increases the UAA's uptake rate and intracellular concentration, thus reducing the amount of UAA required.