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Quantitative Proteomics for Epigenetics
Author(s) -
Eberl H. Christian,
Mann Matthias,
Vermeulen Michiel
Publication year - 2011
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000429
Subject(s) - proteomics , computational biology , proteome , quantitative proteomics , epigenetics , histone , biology , chromatin , identification (biology) , bioinformatics , dna , gene , genetics , botany
Mass spectrometry has made many contributions to the chromatin field through the mapping of histone modifications and the identification of protein complexes involved in gene regulation. MS‐based proteomics has now evolved from the identification of single protein spots in gels to the identification and quantification of thousands of proteins in complex mixtures. Quantitative approaches also allow comparative and time‐resolved analysis of post‐translational modifications. An important emerging field is the unbiased interaction analysis of proteins with other proteins, defined protein modifications, specific DNA and RNA sequences, and small molecules. Quantitative proteomics can also accurately monitor whole proteome changes in response to perturbation of the gene expression machinery. We provide an up‐to‐date review of modern quantitative proteomic technology and its applications in the field of epigenetics.