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A DNA Polymerase with Increased Reactivity for Ribonucleotides and C5‐Modified Deoxyribonucleotides
Author(s) -
Staiger Nadine,
Marx Andreas
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000384
Subject(s) - deoxyribonucleotides , nucleobase , polymerase , dna , dna polymerase , nucleotide , biochemistry , chemistry , computational biology , combinatorial chemistry , biology , microbiology and biotechnology , gene
Just do it: Directed evolution enables efficient enzymatic synthesis of catalytically active RNA as well as highly functionalized C5‐modified DNA. The DNA polymerase variant that is proficient in incorporating nucleobase and sugar‐modified nucleotides was identified by a chip‐based protein library screening assay.