Premium
Production of Dehydrogingerdione Derivatives in Escherichia coli by Exploiting a Curcuminoid Synthase from Oryza sativa and a β‐Oxidation Pathway from Saccharomyces cerevisiae
Author(s) -
Katsuyama Yohei,
Ohnishi Yasuo,
Horinouchi Sueharu
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000379
Subject(s) - curcuminoid , gingerol , saccharomyces cerevisiae , escherichia coli , biochemistry , biosynthesis , dna ligase , chemistry , metabolic engineering , atp synthase , yeast , oryza sativa , bioproduction , enzyme , biology , curcumin , gene , chromatography
Gingerol derivatives are bioactive compounds isolated from the rhizome of ginger. They possess various beneficial activities, such as anticancer and hepatoprotective activities, and are therefore attractive targets of bioengineering. However, the microbial production of gingerol derivatives has not yet been established, primarily because the biosynthetic pathway of gingerol is unknown. Here, we report the production of several dehydrogingerdione (a gingerol derivative) analogues from a recombinant Escherichia coli strain that has an “artificial” biosynthesis pathway for dehydrogingerdione that was not based on the original biosynthesis pathway of gingerol derivatives in plants. The system consists of a 4‐coumarate:CoA ligase from Lithospermum erythrorhizon , a fatty acid CoA ligase from Oryza sativa , a β‐oxidation system from Saccharomyces cerevisiae, and a curcuminoid synthase from O. sativa . To our knowledge, this is the first report of the microbial production of a plant metabolite the biosynthetic pathway of which has not yet been identified.