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Precision Control of Cellular Pathways with Light
Author(s) -
Lemke Edward A.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000364
Subject(s) - lysine , computational biology , chemistry , nuclear localization sequence , microbiology and biotechnology , biology , biochemistry , biophysics , amino acid , gene
Caging protein activity makes it possible to control cellular pathways with high spatial and temporal resolution in a noninvasive manner. Gautier et al. have now developed a system to photocontrol the nuclear import of various cargos in mammalian cells. To achieve this, a critical lysine residue in a nuclear localization sequence was replaced by a genetically encoded photocaged lysine in response to the amber codon TAG.
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