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A Chemoenzymatic Route to Diversify Aminolgycosides Enables a Microarray‐Based Method to Probe Acetyltransferase Activity
Author(s) -
Tsitovich Pavel B.,
Pushechnikov Alexei,
French Jonathan M.,
Disney Matthew D.
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000300
Subject(s) - acetyltransferase , escherichia coli , substrate (aquarium) , computational biology , computer science , microarray , coenzyme a , chemistry , biochemistry , combinatorial chemistry , enzyme , information retrieval , biology , gene , reductase , gene expression , acetylation , ecology
Chemoenzymatic synthesis: A chemoenzymatic route for modification of aminoglycosides is disclosed. The significant feature of this approach is the discovery that an aminoglycoside 3‐ N ‐acetyltransferase, AAC(3)‐IV, from Escherichia coli accepts azidoacetyl coenzyme A (AzAcCoA) as a substrate that can be further chemically diversified. This approach was used to develop a carbohydrate microarray method to study modification of aminoglycosides.