A Chemoenzymatic Route to Diversify Aminolgycosides Enables a Microarray‐Based Method to Probe Acetyltransferase Activity | Zendy

Tsitovich Pavel B. | Zendy; Pushechnikov Alexei | Zendy; French Jonathan M. | Zendy; Disney Matthew D. | Zendy

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A Chemoenzymatic Route to Diversify Aminolgycosides Enables a Microarray‐Based Method to Probe Acetyltransferase Activity

Author(s) -

Tsitovich Pavel B.,

Pushechnikov Alexei,

French Jonathan M.,

Disney Matthew D.

Publication year - 2010

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201000300

Subject(s) - acetyltransferase , escherichia coli , substrate (aquarium) , computational biology , computer science , microarray , coenzyme a , chemistry , biochemistry , combinatorial chemistry , enzyme , information retrieval , biology , gene , reductase , gene expression , acetylation , ecology

Chemoenzymatic synthesis: A chemoenzymatic route for modification of aminoglycosides is disclosed. The significant feature of this approach is the discovery that an aminoglycoside 3‐ N ‐acetyltransferase, AAC(3)‐IV, from Escherichia coli accepts azidoacetyl coenzyme A (AzAcCoA) as a substrate that can be further chemically diversified. This approach was used to develop a carbohydrate microarray method to study modification of aminoglycosides.

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