Premium
Modulation of Shank3 PDZ Domain Ligand‐Binding Affinity by Dimerization
Author(s) -
IskenderianEpps Wendy S.,
Imperiali Barbara
Publication year - 2010
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201000246
Subject(s) - pdz domain , ligand (biochemistry) , biophysics , fluorescence , chemistry , binding domain , binding site , biochemistry , receptor , biology , physics , quantum mechanics
A two‐pronged approach using fluorescence‐based probes and chemically controlled dimerization was successfully implemented to study the functional effects of Shank3 PDZ domain ligand binding in vitro. Overall, Shank3 PDZ‐domain dimerization induces an eight‐ to 30‐fold enhancement of PDZ domain binding affinity for the fluorescent probe and the native nonfluorescent GKAP ligand.